P. aeruginosa Biofilms in CF Infection

Phase II studies of nebulised Arikace in CF patients with Pseudomonas aeruginosa infection

RATIONALE Arikace is a liposomal amikacin preparation for aerosol delivery with potent Pseudomonas aeruginosa killing and prolonged lung deposition. OBJECTIVES To examine the safety and efficacy of 28 days of once-daily Arikace in cystic fibrosis (CF) patients chronically infected with P aeruginosa. METHODS 105 subjects were evaluated in double-blind, placebo-controlled studies. Subjects we...

Localized gene expression in Pseudomonas aeruginosa biofilms.

Gene expression in biofilms is dependent on bacterial responses to the local environmental conditions. Most techniques for studying bacterial gene expression in biofilms characterize average values across the entire population. Here, we describe the use of laser capture microdissection microscopy (LCMM) combined with multiplex quantitative real-time reverse transcriptase PCR (qRT-PCR) to isolat...

Increased mutability of Pseudomonas aeruginosa in biofilms.

OBJECTIVES Isolates of Pseudomonas aeruginosa from cystic fibrosis (CF) patients are frequently hypermutable due to selection of mutants with defects in DNA repair genes such as mutS. Since P. aeruginosa grows as a biofilm within the infected CF lung, it is possible that this mode of growth enhances the mutability of the organism thereby increasing the opportunity to derive permanent hypermutat...

Stratified growth in Pseudomonas aeruginosa biofilms.

In this study, stratified patterns of protein synthesis and growth were demonstrated in Pseudomonas aeruginosa biofilms. Spatial patterns of protein synthetic activity inside biofilms were characterized by the use of two green fluorescent protein (GFP) reporter gene constructs. One construct carried an isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible gfpmut2 gene encoding a stable GFP. T...

Eradication therapy for early Pseudomonas aeruginosa infection in CF: many questions still unanswered.